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fgf10  (R&D Systems)


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    Structured Review

    R&D Systems fgf10
    Fgf10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+fgf10/Recombinant+Human+FGF-10+Protein/pm41997918-303-65-67
    Average 96 stars, based on 183 article reviews
    fgf10 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling.
    Article Snippet: Supernatants were removed and crypts were mixed with Matrigel (Corning, USA), transferred to a 24-well plate and incubated at 37 °C for 10 min. Pre-warmed IntestiCultTM Organoid Growth Medium (STEMCELL technologies, #06005, USA) supplemented with 1 mM nicotinamide (Sigma, USA), 200 ng/ml recombinant FGF10 (R&D Systems, USA), 60 ng/ml recombinant Wnt3A (R&D systems, USA), 200 ng/ml gastrin (Sigma, USA), 2 μM A8301 (Tocris, UK) and 10 μM Y-27632 (Selleckchem, USA) in domeshaped solidified gels. .. Supernatants were removed and crypts were mixed with Matrigel (Corning, USA), transferred to a 24-well plate and incubated at 37 °C for 10 min. Pre-warmed IntestiCultTM Organoid Growth Medium (STEMCELL technologies, #06005, USA) supplemented with 1 mM nicotinamide (Sigma, USA), 200 ng/ml recombinant FGF10 (R&D Systems, USA), 60 ng/ml recombinant Wnt3A (R&D systems, USA), 200 ng/ml gastrin (Sigma, USA), 2 μM A8301 (Tocris, UK) and 10 μM Y-27632 (Selleckchem, USA) in domeshaped solidified gels. ..

    Article Title: 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline
    Article Snippet: Control sections were incubated with Heparitinase I (Seikagaku) before proceeding. .. Sections were then treated with 1% BSA/TBS solution for 10 min before incubation with 3 μM recombinant Fgf2 (R&D Systems) and 9 μM recombinant human Fgfr1α(IIIc)-Fc (R&D Systems); 20 nM recombinant Fgf10 (R&D Systems) and 20 nM recombinant human Fgfr2β(IIIc)-Fc (R&D Systems); 30nM recombinant Fgf8b (R&D Systems) and 100 nM recombinant human Fgfr3(IIIc) (R&D Systems) at 4°C overnight. .. Formation of ternary structure was detected by incubation of 1/200 anti-human IgG (Fc-specific) Cy3 (Sigma) in 1% BSA/TBS. n = 5 for wild-type embryos, n = 3 for mutant embryos.

    Recombinant:

    Article Title: Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling.
    Article Snippet: Supernatants were removed and crypts were mixed with Matrigel (Corning, USA), transferred to a 24-well plate and incubated at 37 °C for 10 min. Pre-warmed IntestiCultTM Organoid Growth Medium (STEMCELL technologies, #06005, USA) supplemented with 1 mM nicotinamide (Sigma, USA), 200 ng/ml recombinant FGF10 (R&D Systems, USA), 60 ng/ml recombinant Wnt3A (R&D systems, USA), 200 ng/ml gastrin (Sigma, USA), 2 μM A8301 (Tocris, UK) and 10 μM Y-27632 (Selleckchem, USA) in domeshaped solidified gels. .. Supernatants were removed and crypts were mixed with Matrigel (Corning, USA), transferred to a 24-well plate and incubated at 37 °C for 10 min. Pre-warmed IntestiCultTM Organoid Growth Medium (STEMCELL technologies, #06005, USA) supplemented with 1 mM nicotinamide (Sigma, USA), 200 ng/ml recombinant FGF10 (R&D Systems, USA), 60 ng/ml recombinant Wnt3A (R&D systems, USA), 200 ng/ml gastrin (Sigma, USA), 2 μM A8301 (Tocris, UK) and 10 μM Y-27632 (Selleckchem, USA) in domeshaped solidified gels. ..

    Article Title: Chondroitin sulfates enhances the barrier function of basement membrane assembled by heparan sulfates
    Article Snippet: .. Recombinant FGF10 and FGFR2b were obtained from R&D Systems, Minneapolis, MN. ..

    Article Title: Neuron-derived FGF10 ameliorates cerebral ischemia injury via inhibiting NF-κB-dependent neuroinflammation and activating PI3K/Akt survival signaling pathway in mice
    Article Snippet: .. Recombinant FGF10 (catalogue: 345-FG-025) was purchased from R&D Systems (Minneapolis, MN). .. Antibodies against FGF10 (ab115825), MAP-2 (ab5392), GFAP (ab7260), tubulin (ab179513) and t-PI3K (ab22653) were purchased from Abcam (Cambridge, MA).

    Article Title: 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline
    Article Snippet: Control sections were incubated with Heparitinase I (Seikagaku) before proceeding. .. Sections were then treated with 1% BSA/TBS solution for 10 min before incubation with 3 μM recombinant Fgf2 (R&D Systems) and 9 μM recombinant human Fgfr1α(IIIc)-Fc (R&D Systems); 20 nM recombinant Fgf10 (R&D Systems) and 20 nM recombinant human Fgfr2β(IIIc)-Fc (R&D Systems); 30nM recombinant Fgf8b (R&D Systems) and 100 nM recombinant human Fgfr3(IIIc) (R&D Systems) at 4°C overnight. .. Formation of ternary structure was detected by incubation of 1/200 anti-human IgG (Fc-specific) Cy3 (Sigma) in 1% BSA/TBS. n = 5 for wild-type embryos, n = 3 for mutant embryos.

    Article Title: Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle
    Article Snippet: .. To determine the effects of FGF10 and FGF22 on granulosa cell mRNA expression and oestradiol production, graded doses of human recombinant FGF10 (0, 1, 10 and 100 ngmL 1; R&D Systems, Minneapolis, MN, USA; 92% homology between human and bovine nucelotide sequences) or FGF22 (0, 1, 10, 100 and 200 ngmL 1; R&D Systems; 90% homology between human and bovine nucelotide sequences) were added to the culture medium from Day 2 of culture. ..

    Article Title: Chondroitin sulfate enhances the barrier function of basement membrane assembled by heparan sulfate.
    Article Snippet: .. Recombinant FGF10 and FGFR2B were obtained from R&D Systems. .. Basement membrane assembly assay C2C12 cells (CRL-1772, American Type Culture Collection) were authenticated and tested for contamination by the vendor and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (10-013- CV, Corning) containing 10% FBS.

    Article Title: Downregulation of ten-eleven translocation-2 triggers epithelial differentiation during organogenesis.
    Article Snippet: .. Isolated epithelia were placed in 20 μL of laminin-111 (4.8 mg/mL; Trevigen, Gaithersburg, MD, USA) and cultured on 0.1-μm membrane filters (GE Healthcare, Piscataway, NJ, USA) in 200 μL of Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with 50 μg/mL transferrin (Merck, Darmstadt, Germany) and 800 ng/mL recombinant FGF10 (R&D Systems, Minneapolis, MN, USA). .. For RNA interference analysis of the isolated epithelium, antisense oligonucleotide (GapmeR; Qiagen, Hilden, Germany) was transfected into the isolated epithelia using the HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (n = 3 biological samples, 4–5 epithelia/sample).

    Expressing:

    Article Title: Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle
    Article Snippet: .. To determine the effects of FGF10 and FGF22 on granulosa cell mRNA expression and oestradiol production, graded doses of human recombinant FGF10 (0, 1, 10 and 100 ngmL 1; R&D Systems, Minneapolis, MN, USA; 92% homology between human and bovine nucelotide sequences) or FGF22 (0, 1, 10, 100 and 200 ngmL 1; R&D Systems; 90% homology between human and bovine nucelotide sequences) were added to the culture medium from Day 2 of culture. ..

    Isolation:

    Article Title: Downregulation of ten-eleven translocation-2 triggers epithelial differentiation during organogenesis.
    Article Snippet: .. Isolated epithelia were placed in 20 μL of laminin-111 (4.8 mg/mL; Trevigen, Gaithersburg, MD, USA) and cultured on 0.1-μm membrane filters (GE Healthcare, Piscataway, NJ, USA) in 200 μL of Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with 50 μg/mL transferrin (Merck, Darmstadt, Germany) and 800 ng/mL recombinant FGF10 (R&D Systems, Minneapolis, MN, USA). .. For RNA interference analysis of the isolated epithelium, antisense oligonucleotide (GapmeR; Qiagen, Hilden, Germany) was transfected into the isolated epithelia using the HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (n = 3 biological samples, 4–5 epithelia/sample).

    Cell Culture:

    Article Title: Downregulation of ten-eleven translocation-2 triggers epithelial differentiation during organogenesis.
    Article Snippet: .. Isolated epithelia were placed in 20 μL of laminin-111 (4.8 mg/mL; Trevigen, Gaithersburg, MD, USA) and cultured on 0.1-μm membrane filters (GE Healthcare, Piscataway, NJ, USA) in 200 μL of Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with 50 μg/mL transferrin (Merck, Darmstadt, Germany) and 800 ng/mL recombinant FGF10 (R&D Systems, Minneapolis, MN, USA). .. For RNA interference analysis of the isolated epithelium, antisense oligonucleotide (GapmeR; Qiagen, Hilden, Germany) was transfected into the isolated epithelia using the HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (n = 3 biological samples, 4–5 epithelia/sample).

    Membrane:

    Article Title: Downregulation of ten-eleven translocation-2 triggers epithelial differentiation during organogenesis.
    Article Snippet: .. Isolated epithelia were placed in 20 μL of laminin-111 (4.8 mg/mL; Trevigen, Gaithersburg, MD, USA) and cultured on 0.1-μm membrane filters (GE Healthcare, Piscataway, NJ, USA) in 200 μL of Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with 50 μg/mL transferrin (Merck, Darmstadt, Germany) and 800 ng/mL recombinant FGF10 (R&D Systems, Minneapolis, MN, USA). .. For RNA interference analysis of the isolated epithelium, antisense oligonucleotide (GapmeR; Qiagen, Hilden, Germany) was transfected into the isolated epithelia using the HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (n = 3 biological samples, 4–5 epithelia/sample).

    Modification:

    Article Title: Downregulation of ten-eleven translocation-2 triggers epithelial differentiation during organogenesis.
    Article Snippet: .. Isolated epithelia were placed in 20 μL of laminin-111 (4.8 mg/mL; Trevigen, Gaithersburg, MD, USA) and cultured on 0.1-μm membrane filters (GE Healthcare, Piscataway, NJ, USA) in 200 μL of Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with 50 μg/mL transferrin (Merck, Darmstadt, Germany) and 800 ng/mL recombinant FGF10 (R&D Systems, Minneapolis, MN, USA). .. For RNA interference analysis of the isolated epithelium, antisense oligonucleotide (GapmeR; Qiagen, Hilden, Germany) was transfected into the isolated epithelia using the HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (n = 3 biological samples, 4–5 epithelia/sample).



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    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or <t>FGF10</t> from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001
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    Image Search Results


    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or FGF10 from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001

    Journal: Stem Cell Research & Therapy

    Article Title: A serum-free culture medium for long-term expansion of human airway basal stem cells

    doi: 10.1186/s13287-026-04910-z

    Figure Lengend Snippet: Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or FGF10 from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001

    Article Snippet: The complete sfBSC medium was supplemented with the following components: 1X B27 supplement (Gibco, 17504044), 0.1% recombinant human serum albumin (rHSA, Sigma-Aldrich, A9731), 2 mM Glutamax (Gibco, 35050061), 50 μg/mL L-ascorbic acid (Sigma-Aldrich, A4544), 0.4 μM monothioglycerol (MTG, Sigma-Aldrich, M6145), 1 μg/mL hydrocortisone (STEMCELL Technologies, 07926), 1 ng/mL recombinant human EGF (Sigma-Aldrich, SRP3027), 0.4 ng/mL recombinant human FGF10 (R&D systems, 345-FG), 10 μM Y27632 (Rho-associated protein kinase (ROCK) inhibitor, Selleck, S1049), 1 μM DAPT (γ-secretase/Notch signaling pathway inhibitor, Sigma-Aldrich, D5942), 1 μM A83-01 (ALK5 inhibitor, Sigma-Aldrich, SML0788), and 1 μM DMH-1 (BMP signaling inhibitor, Sigma-Aldrich, D8946).

    Techniques: Control, CCK-8 Assay, Single Cell

    Optimization of EGF and FGF10 concentrations for maintaining BSC morphology. A Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of EGF. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. B Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of FGF10. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. C Immunofluorescence staining of P63 and KRT5 in HBECs grown in medium supplemented with 40 ng/mL FGF10. Scale bar = 50 μm. D Immunofluorescence staining of NGFR in HBECs grown in media supplemented with 40 ng/mL FGF10. White borders demarcate regions with altered NGFR expression patterns. Scale bar = 50 μm

    Journal: Stem Cell Research & Therapy

    Article Title: A serum-free culture medium for long-term expansion of human airway basal stem cells

    doi: 10.1186/s13287-026-04910-z

    Figure Lengend Snippet: Optimization of EGF and FGF10 concentrations for maintaining BSC morphology. A Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of EGF. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. B Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of FGF10. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. C Immunofluorescence staining of P63 and KRT5 in HBECs grown in medium supplemented with 40 ng/mL FGF10. Scale bar = 50 μm. D Immunofluorescence staining of NGFR in HBECs grown in media supplemented with 40 ng/mL FGF10. White borders demarcate regions with altered NGFR expression patterns. Scale bar = 50 μm

    Article Snippet: The complete sfBSC medium was supplemented with the following components: 1X B27 supplement (Gibco, 17504044), 0.1% recombinant human serum albumin (rHSA, Sigma-Aldrich, A9731), 2 mM Glutamax (Gibco, 35050061), 50 μg/mL L-ascorbic acid (Sigma-Aldrich, A4544), 0.4 μM monothioglycerol (MTG, Sigma-Aldrich, M6145), 1 μg/mL hydrocortisone (STEMCELL Technologies, 07926), 1 ng/mL recombinant human EGF (Sigma-Aldrich, SRP3027), 0.4 ng/mL recombinant human FGF10 (R&D systems, 345-FG), 10 μM Y27632 (Rho-associated protein kinase (ROCK) inhibitor, Selleck, S1049), 1 μM DAPT (γ-secretase/Notch signaling pathway inhibitor, Sigma-Aldrich, D5942), 1 μM A83-01 (ALK5 inhibitor, Sigma-Aldrich, SML0788), and 1 μM DMH-1 (BMP signaling inhibitor, Sigma-Aldrich, D8946).

    Techniques: Cell Culture, Immunofluorescence, Staining, Expressing